Cisplatin h460

Author: hdmx

August undefined, 2024

WebC is-diamminedichloroplatinum(II) (cisplatin) is the most celebrated metal-based chemotherapeutic in the history of modern medicine.The discovery of its anticancer effects was not only opportune [1,2,3], especially during a period when cancer prevention was desperately needed, but also because medical oncology did not yet exist as a clinical … WebApr 9, 2024 · In vitro cytotoxicity of free cisplatin and G4.0-PAA/aquated cisplatin nano-complex against NCI H460 cells (human lung cancer cells) was evaluated by SRB assay, and their corresponding IC 50 values were calculated from the dose–response curves using non-linear regression analysis. The cells were incubated for 5 h at pH 7.4 in the …

Cisplatin-induced hydroxyl radicals mediate pro-survival …

WebApr 9, 2024 · However, in the H460 cell line with the low basal expression level of SNRPB, overexpressing of SNRPB markedly increased cisplatin-induced cell proliferation … WebMay 1, 2024 · Cisplatin-resistant versions of the A549, H1299, and H460 cell lines were generated by continuous drug exposure. The long-term metabolic changes, as well as, the early response to cisplatin treatment were analyzed in both, parental and cisplatin-resistant cell lines. how many islands make up bermuda

Long-term cisplatin exposure impairs autophagy and causes cisplatin …

WebFeb 28, 2024 · One of the main problems in chemotherapy using platinum drugs as anticancer agents is the resistance phenomenon. Synthesizing and evaluating valid alternative compounds is challenging. This review focuses on the last two years of progress in the studies of platinum (II)- and platinum (IV)-based anticancer complexes. In … WebResistance mechanism to cisplatin in NCI-H460 non-small cell lung cancer cell line: investigating apoptosis, autophagy, and cytogenetic damage … WebFlap endonuclease 1 (FEN1) plays a crucial role in both DNA replication and damage repair. In this study, we investigated FEN1 expression and its clinical-pathological significance in non-small-cell lung cancer (NSCLC). Quantitative reverse PCR and how many islands make up fiji

Cisplatin resistance involves a metabolic reprogramming

Generation and Characterisation of Cisplatin-Resistant Non-Small …

WebCISPLATIN 10 MG INJECTION: Short descriptive text of procedure or modifier code (28 characters or less). The AMA owns the copyright on the CPT codes and descriptions; … WebJan 26, 2024 · Emodin enhances cisplatin-induced DNA damage in A549 and H460 cells Cisplatin mainly kills tumor cells by inducing DNA damage ( 30 ). The effect of emodin on the chemosensitivity of A549 and H460 … how many islands make up great britainWebSep 1, 2006 · Figure 2C shows that H460-pMEP 4-MKP-1 cells treated with CdSO 4 were more resistant to both doses of cisplatin compared with the control H460-pMEP 4 vector cells . Thus, these results indicate that overexpression of MKP-1 plays a critical role in cisplatin resistance in the H460 human lung cancer cell. how many islands make up the channel islands

"WebDec 16, 2015 · H460 lung cancer cells increased cisplatin sensitivity and showed reduced repair of both . intrastrand and interstrand adducts suggesting that the mechanism of sensitivity is primarily . " - Cisplatin h460

Cisplatin h460

Web用 BA 对 H460 细胞进行处理，采用 MTT 法检测细胞增殖情况，并利用 Annexin V-FITC/PI 双染法检测 BA 对 H460 细胞凋亡的影响。Hoechst 33342 染色和免疫荧光染色观察细胞凋亡时染色质的凝集以及 DNA 损伤标志蛋白 γ-H2AX 表达情况。 WebMay 28, 2024 · Cells endogenously expressing high (A549 and H460 cells) versus low (EBC-1, CRL-5889 and HTB-182 cells) CDCA3 levels were untreated, cisplatin treated for 12 h (cisplatin) or cultured in...

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WebJun 20, 2024 · H460-Cis and A549-Cis cells were treated with 2 μ g/mL cisplatin to maintain cisplatin resistance character. All human cell lines have been authenticated using short tandem repeat (STR) profiling. Cells were maintained at 37°C in a humidified atmosphere with 5% CO 2. Web2 days ago · LUAD cell lines H1299, H460, A549, H1975, CALU1 and CALU6, lung fibroblasts HEL299, lung bronchial epithelial cell line HBEC, normal human bronchial epithelium cell line BEAS2B, kidney embryonic ...

WebApr 14, 2024 · To verify the resistance of A549/CR and H460/CR cells, the effect of cisplatin or PGG on viability (Figure 1A) and the PTEN expression level was examined in A549 and H460 parental cells and cisplatin-resistant A549/CR and H460/CR cells using an MTT assay and Western blotting. Cisplatin-resistant cell lines demonstrated a subtle … WebJul 26, 2024 · Expression profiling of cisplatin resistant cells derived from H460 lung cell line. Organism: Homo sapiens: Experiment type: Expression profiling by array: …

Web检测H460细胞中p-Akt蛋白的表达。将细胞接种于6孔板中，加入不同浓度(10、20、30 μmol/L)芝麻素处理12 h后，在每孔细胞中加入100 μL的蛋白裂解液后，采用BCA试剂盒测定蛋白浓度，计算每个泳道的上样量为70 μg总蛋白，计算5× loading buffer量及超纯水量，定 … WebJan 26, 2012 · The cisplatin resistant clone (H460/cis) of H460 cells was established by exposing the cells with gradually increasing concentrations of cisplatin until chemoresistance acquisition was elucidated by MTT, Hoechst 33342 staining and comet assays. Degree of autophagosome formation and level of LC3 marker were evaluated by …

WebJul 6, 2024 · A2780 and H460 cells were treated with increasing concentrations of cisplatin (Hospira, Australia) for 48 h, collected, washed twice by centrifugation at 900 g for 5 min with FACS buffer (2.5 % FCS, 0.04 % sodium azide in PBS) and incubated with 5 μg/mL chDAB4 or human IgG (Intragram P; as control) for 30 min. Cells were washed and …

WebConversely, H460 cisplatin-resistant cells showed increased tendency to develop bone metastasis. Gene expression analysis showed that several genes involved in tumor … how many islands make up singaporeWebJul 28, 2024 · Cisplatin induces an autophagic response in lung cancer H460 cells. To investigate the autophagic response, the alteration of autophagy marker proteins was … how many islands make up comorosWebFeb 4, 2024 · H460 cells showed a greater sensitivity to cisplatin with 41% inhibition of colony formation at 1 µM cisplatin and 80% inhibition at 2 µM, compared with 23% and 32%, respectively, for A549... howard integrity gisWebMay 11, 2012 · Cisplatin ( cis -diamminedichloroplatinum) is a platinum-based agent, which has been wildly utilized in systemic therapeutic regimens for lung cancer, both NSCLC and small-cell lung cancer. 5... howard insurance agency roanoke vaWebCisplatin may cause severe kidney problems, nerve problems, and severe nausea and vomiting. Nerve problems may occur during treatment or weeks after stopping treatment. howard international affairsWebThe IC 50 concentrations detected in A549 and H460 cisplatin-resistant cell lines and the parental cell lines. Source publication ABCG2 regulates the pattern of self-renewing … how many islands make up palauWebOct 31, 2024 · Generation of Cisplatin-Resistant Lung Cancer Cell Lines A549, H460, H661, and H1299 lung cancer cells were exposed to cisplatin according to the IC50 value for 72 h. The medium was removed, and the cells were cultured in normal medium until the cells fully recovered. howard international house