Fitc active caspase-3 apoptosis kit
WebThe Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product. The fluorescence intensity (λEx = 400 nm/λEm = 490 nm) is proportional to the caspase-3 activity. WebFITC-C6-DEVD-FMK is a non-toxic, cell-permeable fluorescent substrate that irreversibly binds to activated caspase-3 in apoptotic cells. The FITC-labeled substrate can be detected with high sensitivity using flow cytometry. Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins ...
Fitc active caspase-3 apoptosis kit
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WebCaspases are cysteine proteases that can cleave other proteins, and upon activation, create a positive-feedback cascade, which ensures the cell will undergo apoptosis. Our … WebFITC-DEVD-FMK is cell permeable, non-toxic, and irreversibly binds to activated Caspase-3 in apoptotic cells. The FITC label allows detection …
WebImage-iT LIVE Caspase-3 and -7 Detection Kits; Application: No-wash assay to monitor ongoing caspase-3 and -7 activity. End point assay for the detection of active caspase-3 and -7. Readout: Following interaction with active caspase-3 and -7 in apoptotic cells, the fluorogenic, cell-permeant substrate is able to bind DNA and fluoresce brightly. WebProduct overview. Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy. The kit can differentiate apoptosis vs ...
WebApr 15, 2024 · Cells were washed with PBS, trypsinized, and stained for active caspase-3 following the protocol using the FITC Active Caspase-3 Apoptosis Kit (BD550480) according to the manufacturer’s ... WebCaspase-3 (6) Apoptosis Antibody Array (1) ... Active Caspase-3/7 Staining Kit. Reactivity: Human. ... Cleaved Caspase-3 Staining Kit (FITC) (ab65613) Reviews (1) Specific References (21) Description: Cleaved Caspase-3 Staining Kit (FITC) Sample type: Adherent cells, Suspension cells.
WebJul 22, 2012 · Caspase-3 activation was determined using the FITC Active Caspase-3 Apoptosis kit (BD Pharmingen, San Diego, CA, USA) according to the manufacturer's instructions. The cells were resuspended in BD Cytofix/Cytoperm buffer in the provided solution at a concentration of 2 × 10 6 cells/mL and were incubated 20 min in ice.
WebSpecificity. Caspase-3 is a member of the interleukin-1 β-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating two subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal ... in floor heating boiler systemsWebBD Caspase-3, Active Form, mAb Apoptosis Kit : FITC (CPP32) For Intracellular staining Manufacturer: BD 550480 Catalog No. BDB550480 … in floor heating glycolWebNov 7, 2005 · By contrast, caspase-8 inhibitor (z-IETD-fmk) had no effect, whereas the same concentration of this inhibitor completely blocked FasL-induced apoptosis in HSFs (unpublished data). Immunoblot analysis showed that both caspase-9 and -3 were cleaved into their active forms upon CCN1 treatment (Fig. 5 B). in floor heat boilers pricesWebJan 31, 2012 · This product is manufactured by BioVision, an Abcam company and was previously called K182 CaspGLOW™ Fluorescein Active Caspase-2 Staining Kit. K182-100 is the same size as the 100 test size of ab65612. Activation of caspases plays a central role in apoptosis. Other caspase and apoptosis assays in floor heating flooring optionsWebBecton Dickinson fitc active caspase 3 apoptosis kit Fitc Active Caspase 3 Apoptosis Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, … in floor heating filmWebActive caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 … in floor heating laminate flooringWebThe CaspGLOW™ Fluorescein Active Caspase-9 Staining Kit contains all the reagents necessary to detect active caspase-9 in mammalian cells with high sensitivity. Fluorescein (FITC)-conjugated LEHD-FMK, a specific inhibitor of caspase-9, is utilized in this assay for detection. This reagent is cell permeable, non-toxic and irreversibly binds to ... in floor heating for tile