WebbAfter transfection, the HBV-DNA quantitative kit (2024005; DAAN Gene, Guangzhou, China), including the reagentsforDNAextraction,PCRamplification,calibration,andqualitycontrol,wasused for measuring the level of HBV-DNA in one mL of cell culture supernatant, as per … Webb14 okt. 2014 · A, The IC 50 dosed drug-triggered cell death of 5637 cells transfected by miR-193a-3p mimic (3 PM) or the gene specific siRNAs versus the negative control (NC). B, The levels by qRT-PCR of both LOXL4 and SRSF2 mRNAs in the 3 PM, or siRNAs (single or in combination) transfected 5637 cells and the 3PA transfected H-bc cells versus the NC.
Frontiers Potential Novel Prediction of TMJ-OA: MiR-140-5p …
WebbAdditionally, to silence the expression of circFAM114A2 and HHIP, two small interfering RNAs (siRNA) were synthesized by RiboBio (China). The transfection of siRNAs, miR-630 mimic, and scramble controls were performed using the riboFECT CP Transfection Kit (Ribobio, C10511-05). All siRNA sequences are shown in Table S1. Webb18 jan. 2024 · The siRNA oligonucleotides for mouse COL1A1 and siRNA control were purchased from Ribobio. MC3T3‐E1 and Raw264.7 cells were transfected using a RiboFECT CP Transfection Kit (RiboBio), followed by validation of mRNA change at 36 h after transfection using qPCR and protein change at 48 h using western blotting. drywall pricing per square foot
miR-193a-3p regulates the multi-drug resistance of bladder cancer …
Webb7 sep. 2016 · RiboFECT CP Transfection kit (166T) (RiboBio) was used for the miR-671 inhibitor and mimic. Detection of cell apoptosis by FCM The Annexin V-fluorescein … Webb13 mars 2024 · 转染方法 以riboFECT? CP Reagent 转染siRNA于24孔板,转染浓度为50nM为例,其他规格容器转染请参考表2。. 1. 接种细胞 a. 贴壁细胞:转染前一天,接种1×105细胞至含有适量完全培养基的24孔板培养孔中,使转染时的细胞密度能够达到50~80%。. 注:1)不同细胞生长速度不 ... WebbRibofect Cp Transfection Kit 166 T Ribobioco, supplied by Ribobio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … commerce township property records