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Ribofect cp transfection kit 166t

WebbAfter transfection, the HBV-DNA quantitative kit (2024005; DAAN Gene, Guangzhou, China), including the reagentsforDNAextraction,PCRamplification,calibration,andqualitycontrol,wasused for measuring the level of HBV-DNA in one mL of cell culture supernatant, as per … Webb14 okt. 2014 · A, The IC 50 dosed drug-triggered cell death of 5637 cells transfected by miR-193a-3p mimic (3 PM) or the gene specific siRNAs versus the negative control (NC). B, The levels by qRT-PCR of both LOXL4 and SRSF2 mRNAs in the 3 PM, or siRNAs (single or in combination) transfected 5637 cells and the 3PA transfected H-bc cells versus the NC.

Frontiers Potential Novel Prediction of TMJ-OA: MiR-140-5p …

WebbAdditionally, to silence the expression of circFAM114A2 and HHIP, two small interfering RNAs (siRNA) were synthesized by RiboBio (China). The transfection of siRNAs, miR-630 mimic, and scramble controls were performed using the riboFECT CP Transfection Kit (Ribobio, C10511-05). All siRNA sequences are shown in Table S1. Webb18 jan. 2024 · The siRNA oligonucleotides for mouse COL1A1 and siRNA control were purchased from Ribobio. MC3T3‐E1 and Raw264.7 cells were transfected using a RiboFECT CP Transfection Kit (RiboBio), followed by validation of mRNA change at 36 h after transfection using qPCR and protein change at 48 h using western blotting. drywall pricing per square foot https://foreverblanketsandbears.com

miR-193a-3p regulates the multi-drug resistance of bladder cancer …

Webb7 sep. 2016 · RiboFECT CP Transfection kit (166T) (RiboBio) was used for the miR-671 inhibitor and mimic. Detection of cell apoptosis by FCM The Annexin V-fluorescein … Webb13 mars 2024 · 转染方法 以riboFECT? CP Reagent 转染siRNA于24孔板,转染浓度为50nM为例,其他规格容器转染请参考表2。. 1. 接种细胞 a. 贴壁细胞:转染前一天,接种1×105细胞至含有适量完全培养基的24孔板培养孔中,使转染时的细胞密度能够达到50~80%。. 注:1)不同细胞生长速度不 ... WebbRibofect Cp Transfection Kit 166 T Ribobioco, supplied by Ribobio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … commerce township property records

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Category:LEF-1 Regulates Tyrosinase Gene Transcription In Vitro - PLOS

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Ribofect cp transfection kit 166t

EphA5 knockdown enhances the invasion and migration ability of ...

Webb1 juli 2024 · Similarly, a total of 100 nmol/L of a miR-551b-5p inhibitor (Ribobio, CA, China) was introduced using the riboFECT ™ CP transfection kit (Ribobio, CA, China) according to the manufacturer's protocol. HUVECs were plated onto collagen-coated plates with silicone elastomer bottoms (Flexcell, Mckeesport, PA, USA). WebbRibofecttm Cp Transfection Kit 166t, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Ribofect cp transfection kit 166t

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Webbwherein the double-stranded RNAi agent comprises a sense strand and an antisense strand forming a double stranded region, the antisense strand comprising a region of complementari Webb2 aug. 2024 · Transfection of miR-96 mimetics and mimetic controls were performed with riboFECT CP Transfection Kit (RiboBio) according to the manufacturer’s instruction. The final concentration of...

Webb22 okt. 2024 · The cells were transfected in triplicate with each firefly luciferase reporter construct in combination with the Renilla luciferase-based control construct using the riboFECT CP transfection reagent, and both the luciferase activities were measured in the cell extracts 24 h after transfection. Webb13 mars 2024 · riboFECT? CP 转染试剂使用说明 RN:R10035.3 产品简介 riboFECT? CP 是一种专门用于核酸分子的转染试剂,极具生物相容性,转染效率高,细胞毒性小,适合 …

Webb26 rader · 8 aug. 2024 · 英文名称: ribo FECT ™ CP Transfection 产品货号: C10511-05、C10511-1 产品规格: 166T、333T 产品产地: 广州 产品商标: ribobio 价 格: 询价 保 … http://img1.bioon.com/reagent/Show_product.asp?id=4430241

Webb166T 产品描述 ribo FectTM CP转染试剂是一种专门用于核酸分子的转染试剂,极具生物相容性,转染效率高,细胞毒性小,适合各种核酸分子 (如siRNA、miRNA、质粒)的转染 …

WebbRibobio co c10511 05 ribofect cp transfection kit 166t C10511 05 Ribofect Cp Transfection Kit 166t, supplied by Ribobio co, used in various techniques. Bioz Stars … commerce township property tax ratesWebbtitative real-time PCR using miDETECT A Track Kit (RiboBio) and performed on the ABI 7500 System (Applied Biosystems). The primers for miR-20a-5p and U6 sm all nuclear … commerce township police depthttp://img1.bioon.com/reagent/cpxj3.asp?id=4430241 dry wall projector weightWebbriboFECT™ CP转染试剂是一种专门用于核酸分子的转染试剂,极具生物相容性,转染效率高,细胞毒性小,适合各种核酸分子(如siRNA、miRNA、质粒)的转染实验。该转染试剂转染时无需进行培养基更换操作,尤其适合各种高通量细胞筛选实验,方便,快速,高效。 drywall primer on woodWebb10 jan. 2024 · The above oligonucleotides products transfected H9c2 cells for 24 h by using the riboFECT CP Transfection Kit (RiboBio, China) as directed by the protocol of the manufacturer. Briefly, the oligonucleotides were diluted with 1× riboFECT CP buffer, then added with riboFECT CP reagent and mixed gently. drywall primer how many coatsWebb14 aug. 2024 · Melanocyte transfections were performed using riboFECT™ CP (Ribobio) in accordance with the manufacturer’s protocol. Approximately 5 μL of 20 μM miRNA … commerce township post office 48382WebbCell transfection. HOXC-AS2 small interfering RNA (siHOXC-AS2) and control siRNA (siCtrl), ZEB1 small interfering RNA (siZEB1) and control siRNA ... The ORF region of ZEB1 cDNA was insert into pcDNA3.1 plasmid (ZEB1). RiboFECT CP Transfection Kit (RiboBio, Guangzhou, China) was purchased for transient transfection according to … drywall primer over paint