Top/fop reporter assay

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August undefined, 2024

Web目的. 研究VPS26在高脂环境中对大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSC）成骨、成脂分化作用的机制，探讨VPS26对高脂大鼠种植体骨结合和裸鼠异位成骨的影响。 Web2. apr 2024 · 胃癌细胞系MGC-803购自ATCC；SGC-7901细胞购自中国科学院细胞库；抗PRRX2、β-catenin、c-Myc、cyclin D1和GAPDH I抗以及HRP标记的山羊抗兔Ⅱ抗均购自ProteinTech；转染试剂脂质体Lipofectamine 3000(Invitrogen)；胰蛋白酶(索莱宝生物科技有限公司)；MTT细胞活力检测试剂盒(碧云天生物 ...

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Web2. apr 2013 · TamR cells exhibited increased Wnt signalling as measured via the TOP/FOP Wnt luciferase reporter assays. Genes associated with both the β-catenin dependent (AXIN2, MYC, CSNK1A1) and independent arms (ROR2, JUN), as well as general Wnt secretion (PORCN) of the Wnt signalling pathway were upregulated in the TamR cells compared to … Web15. aug 2013 · Using lentiviral wild-type (Top) and mutant (Fop) TCF/LEF reporters, we investigated the status of Wnt signaling in cells derived from several types of human cancer. As a result of this screen, we identified up-regulated TCF/LEF activity in different hematopoietic tumor cells, including Ramos, K562 and Jurkat ( Figure 1A ). pitbull homeowners insurance

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Web10. jan 2024 · In the present study, the TOP/FOP-Flash reporter assay showed that CDX2 knockdown or CDX2 overexpression led to enhanced or attenuated Wnt signaling activity in colon cancer cells. WebPlasmid TOP-GFP from Dr. Ramesh Shivdasani's lab contains the insert 7xTOP-GFP and is published in Cancer Res. 2012 Feb 8. This plasmid is available through Addgene. ... FOP-GFP TOP-GFP.mC Lentiviral-TOP-dGFP-reporter FOP-GFP.mC M50 Super 8x TOPFlash ... WebSet of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays. Synonym (s): TOPFlash/FOPFlash, TOPFlash plasmid, FOPFlash plasmid. eCl@ss: 32161000. Pricing and availability is not currently … pitbull honorary doctorate

TOPflash (TCF Reporter Plasmid) 21-170 - EMD Millipore

TCF Reporter Plasmid Kit - Sigma-Aldrich

Web12. júl 2024 · The Dual-Luciferase Reporter Assay (Promega) was applied according to the manufacturer’s instructions. For the TOP/FOP-Flash assay, TOP/FOP-Flash (Genechem) was co-transfected into cells along with LUCAT1 silence or overexpression vector, miR-5582-5p mimic or inhibitor, and/or the miRNA control. WebLuciferase reporter assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest. Luciferase genes vary in stability, light output and spectral characteristics, and reporter kits are available with different luciferases for both single- and dual-spectral … pitbull hometownWeb5. dec 2024 · TOP-Flash contained a wild type LEF/TCF-binding site, while FOP-Flash contained a mutant LEF/TCF-binding site. HCT116 cells were transfected with reporter constructs (TOP-Flash or FOP-Flash). pitbull hoodies for dogs

"WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 min. Then, the mixture... " - Top/fop reporter assay

Top/fop reporter assay

Does anyone have experience with the TOPFLASH assay?

WebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay ...

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Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a luciferase reporter gene. fopflash contains mutated tcf binding sites driven by the same … WebLuciferase reporter assays were performed by transfecting TOP/FOP Flash (A), Hes1 (B) or Hey1 (C) luciferase reporter in the cells treated with or without rapamycin (Rapa, 200nM, Sigma), an ...

WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. FOPFlash is similar, except that the Tcf-binding sites are mutated and non-functional. … WebПодтвержденная временем надежность профессионалов. Мы работаем в области аудита и консалтинга с 1997 года, когда было создано ЗАО «Аудиторская фирма «ФОРПОСТ», в 2005 году оно было преобразовано в ООО «Аудиторская ...

Web( A ) TOP/FOP luciferase reporter assays of 8988T cells treated with vitamin C (4 or 5 mM) for 24 h. ( B , C ) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars reflect the s.d. of three … WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from various sources including lentivirus-based reporters from the Nusse and Moon labs, … This is a list of target genes of Wnt/beta-catenin signaling. Suggestions for … In cells not exposed to the signal, β-catenin levels are kept low through interactions … Also see the page on Multiple Wnt receptors. Reviews by Jiang and Cong … There is significant interest in finding small molecules that can either activate or … Activates Wnt reporter with norrin . Activates Wnt reporter with Wnt5A and … GSK-3 (zw3 or shaggy in Drosophila) is a key enzyme in Wnt signaling. It … There are various ways to specifically inhibit Wnt signaling, in cell culture … Assays - How to detect and activate Wnt signaling The Wnt Homepage

WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is activated, beta-catenin will translocate to the nucleus to associate with TCF/LEF …

Web14. nov 2024 · The re-localization of β-catenin prompted us to investigate Wnt-dependent transcription by using the Dual-luciferase reporter system and TOP/FOP reporter assays system. Upon knock-down of H19, the TOP/FOP ratio decreased to about 50% when compared to the control in both MCF-7-R and SK-BR-3-R cells ... stickers for guitar bodyWeb22. sep 2024 · The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously. It has broad applications across various fields of cell and molecular biology—wherever you want to … pit bull hoodie sweatshirtsWebHello, I recently started using the TOPFlash assay and I want a positive control to make sure the vectors are working. I read several places that LiCl can be used to activate Wnt signaling. pitbull hondWeb12. apr 2024 · The global Reporter Gene Assay market size is projected to grow from USUSD 1344.7 million in 2024 to USUSD 2010.3 million in 2029; it is expected to grow at a CAGR of 5.9Percent from 2024 to 2029 ... stickers for instax cameraWeb28. máj 2015 · Dual-luciferase reporter system and TOP/FOP reporter assays confirmed that TP53 GOF mutants were able to enhance the transcriptional ability of Wnt signaling. An exclusive relationship between the status of TP53 and CTNNB1 mutations was observed. stickers for gaming pc of minecraftWebA, B, C. Western blot analysis and TOP/FOP Flash reporter assays confirmed that TP73-AS1/miR-27b-3p/TMED5 axis affected wnt/β-catenin pathway. D. Representative images of the tumor lumps of each ... pitbull hotel room albumWebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 … pitbull hotel holiday inn